Fig. 4.

Bafilomycin A, an autophagy inhibitor, diminishes curcumin-induced cytotoxicity. Cells were treated with a range of curcumin with or without NAC or bafilomycin A. (A) Cell viability was determined by WST-8 assay. Data are expressed as the mean±SD of three independent experiments conducted in triplicate (^*p<0.05 control vs 10 mM NAC, and control vs 50µM bafilomycin A). (B) Western blot analysis was conducted to detect LC3 and SQSTM1 protein. GAPDH was used as a loading control. The data shown are representative of three independent experiments.