Figure 2.

Quantitative line profile analysis of wave splitting. Line profiles of the same cell at eight time points are shown as pixel intensity (0–255 gray levels) vs. pixel number. The intensity of each point was determined by summing the intensities for all pixels in a row (the pixel rows were perpendicular to the direction of cell orientation). Data were obtained from micrographs collected for 0.1 μs. Each panel is separated by 80 ms. Wave splitting is observed in C. The wave intensities are reduced in D–G. The waves overlap in J. (Bar = 100 gray levels.)