Table 5.
Comparison between cDNA microarray analysis and real-time RT-PCR.
| Genbank No. | Gene | cDNA microarray | Real time RT-PCR | ||
|---|---|---|---|---|---|
| HDcon | HDC | HDcon | HDC | ||
| BG085346 | Acetyl-Coenzyme A acyltransferase 2 (Acaa2) | −4.18 | ↔ (1) | 0.28(2) | 0.94 |
| AA277495 | Carnitine palmitoyltransferase 1a, liver (Cpt1a) | ND(3) | ND | 1.73 | 3.41 |
| BF457090 | 24-dehydrocholesterol reductase (Dhcr24) | 2.1 | ↔ | 1.45 | 0.78 |
| BG063933 | Dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (Dpagt1) | 2.3 | ↔ | 3.02 | 1.91 |
| AA023077 | Hydroxysteroid 11-beta dehydrogenase 1 (Hsd11b1) | −8.95 | ↔ | 0.23 | 1.14 |
| AI835105 | Monoglyceride lipase (Mgll) | −8.99 | ↔ | 0.29 | 0.62 |
| W82212 | Phytanoyl-CoA hydroxylase (Phyh) | −5.78 | ↔ | 0.37 | 1.21 |
| AI893897 | Paraoxonase 1 (Pon1) | 2.0 | ↔ | 1.68 | 0.69 |
| AA259329 | Protein kinase, AMP-activated, gamma 1 noncatalytic subunit (Prkag1) | 2.2 | ↔ | 2.20 | 1.41 |
| AA108401 | Solute carrier family 27 (fatty acid transporter), member 2 (Slc27a2) | −4.06 | ↔ | 0.25 | 0.86 |
| AA254935 | Solute carrier family 27 (fatty acid transporter), member 5 (Slc27a5) | −11.73 | ↔ | 0.25 | 0.83 |
(1)The symbol double-headed arrow (↔) denotes that there is no significant difference when gene expression in HDcon or HDC groups was compared with the NDcon group. (2)Data are expressed as fold changes, normalized to β-actin mRNA expression, where the values for the NDcon mice were set at 1.00. The analyses were performed in duplicate. (3)ND, no detected.