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. 2011 Mar 3;2011:235176. doi: 10.4061/2011/235176

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Copyright © 2011 Jeffery J. Auletta et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of time-in-culture on the immunosuppressive activity of hMSCs. Immunosuppressive activity in conditioned medium from IL-1β-stimulated hMSCs at different passages cultured under control conditions. Values are normalized to the inhibition observed conditioned medium from first passage hMSCs. The mean and standard deviation for all 5 hMSC preparations are displayed in (a); the 3 hMSC preparations that exhibited sustained activity are displayed in (b); the mean and standard deviation for the 2 hMSC preparations that exhibited decreased activity after 4 passages are displayed in (c). Asterisks indicate significant differences in one-tail paired t-test; *P < .05.