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. Author manuscript; available in PMC: 2012 Feb 18.
Published in final edited form as: Mol Cell. 2011 Feb 18;41(4):384–397. doi: 10.1016/j.molcel.2011.01.024

Figure 2. Mapping the interaction regions of WAC and RNF20/40.

Figure 2

(A) SFB-tagged wild type WAC and its deletion mutants were expressed in 293T cells. Cell lysates were IPed and blotted with the indicated antibody. The expression level of exogenous WAC was blotted with anti-FLAG antibody from whole cell lysates (WCL). (B–C) A coiled-coil region of RNF20 (B) or RNF40 (C) interacts with WAC. HA-tagged wild type RNF20 or RNF40 and their internal deletion mutants were expressed in 293T cells. The interaction between RNF20/40 and WAC were IPed and blotted with the indicated antibodies.