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. Author manuscript; available in PMC: 2012 Feb 18.
Published in final edited form as: Mol Cell. 2011 Feb 18;41(4):384–397. doi: 10.1016/j.molcel.2011.01.024

Figure 7. WAC is important for G1/S checkpoint activation in response to DNA damage.

Figure 7

(A) HCT116 cells were transfected with the indicated siRNA followed by 15 Gy IR treatments. mRNA level of p21 was examined by qPCR 12 hours after IR. Error bars: SD of triplicate experiments. (B) As in (A) except that 12 hours after IR, cells were pulse labeled with 20 µM BrdU for 30 minutes and stained with FITC-conjutated anti-BrdU and PI. Incorporation of BrdU and total DNA content were determined by FACS. Cells at G1/S transition and early S phase with positive BrdU staining were calculated. (C) A model of WAC mediates transcription-coupled H2B ubiquitination. WAC recognizes RNA polymerase II C-terminal domain and recruits RNF20/40 and RAD6 to gene transcription sites for H2B ubiquitination.