FIGURE 4.
Mitochondrial tRNA sequences from D. discoideum indicate a need for 5′-tRNA editing. Aminoacyl-acceptor stems of the 18 mt-tRNAs encoded in the D. discoideum mitochondrial genome are shown; the remainder of each tRNA sequence is omitted for clarity. Two genes for mt-tRNAIleGAU that differ by only two nucleotides are indicated as IleGAU1/2 as they are identical in their aminoacyl-acceptor stems. The need for mt-tRNA editing is reflected by the presence of non-Watson–Crick unpaired residues in the acceptor stem for the nine tRNAs (including the two IleGAU isoforms) shown on the bottom row. Residues expected to be added by 5′ editing are indicated by arrows to the left of each incorrect nucleotide.