FIGURE 4.

Structure and activity of the wild-type and a mutant glmS ribozyme from T. radiovictrix. (A) The sequence and proposed secondary structure of the glmS ribozyme from T. radiovictrix. A portion of the P1 stem was deleted to yield construct M1, which removes nucleotides 20–52 and changes the loop sequence from GCGA to UAAC. The nucleotide positions within this RNA correspond to the numbering scheme in the wild-type construct. (B) Wild-type ribozyme self-cleavage assays conducted at 50°C as described for Figure 3B. Other annotations are as described for Figure 2B. (C) M1 ribozyme self-cleavage assays conducted as described in B.