Representative integrated phrenic neurograms before, during and 60 min following respiratory neural activity deprivation with 30 min of hypocapnia (A), 30 min of 0.5% isoflurane (B) or 25 min of high frequency ventilation (HFV; C). The white dashed line represents baseline burst amplitude. In all cases, phrenic burst amplitude was increased above baseline within 30 min of returning to respiratory neural activity. Note that in example B, phrenic burst activity did not completely go to zero during isoflurane treatment, suggesting that complete phrenic inactivity is not required for iPMF expression.