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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Blood Cells Mol Dis. 2011 Feb 4;46(3):212–219. doi: 10.1016/j.bcmd.2011.01.001

Figure 5. Chromatin Immunoprecipitation (ChIP) assay for MBD2 binding to the human ε-globin gene promoter region.

Figure 5

(A) ChIP assays with anti-sera for MBD2 and control IgG were performed on adult splenic erythroblasts from wild type or MBD2 homozygous knockout mice transgenic for the β-YAC construct, and immunoprecipitated ε-globin sequences were quantitated by qPCR. Input DNA was assayed to validate primers. (B) ChIP assays showing a positive control using the same MBD2 anti-sera and control IgG used in the experiments in Panel A but with qPCR probes for GATA 2.