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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Dev Cell. 2011 Mar 15;20(3):364–375. doi: 10.1016/j.devcel.2011.01.005

Figure 3. Retrotransposons are derepressed in MitoPLD mutant testes.

Figure 3

A. Quantitative PCR analysis of L1Md and IAP retrotransposon RNAs in P2wk testes. Testes from two MitoPLD-/- mice were analyzed (red and blue bars). The level of Gapdh mRNA was used to normalize the data from mutants and wildtype mice. The relative RNA levels are shown. Error bars represent S. D. (n = 3).

B. In situ hybridization analysis of P16 testes with a probe that detects L1Md RNA. Lump-like signals of L1Md RNA are observed around nuclei of spermatocytes only in MitoPLD-/- testes. Nuclei were stained with Nuclear Fast Red.

C. DNA methylation analysis of retrotransposon sequences in P5-7 spermatogonia by bisulfite sequencing.