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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Mol Microbiol. 2011 Jan 6;79(5):1194–1203. doi: 10.1111/j.1365-2958.2010.07500.x

Figure 3. Lack of peroxidase activity by Clostridium selenoprotein Grx forms.

Figure 3

Peroxidase activity of Sec- and Cys-containing Grxs was measured with H2O2. Bovine erythrocyte Gpx1 was used as positive control for this assay. The decreased absorbance values of samples at 340 nm were subtracted from that of the background without the enzyme. Enzyme activity was calculated using a molar extinction coefficient of NADPH oxidation. Negative activity values were derived from all Grxs samples, indicating no apparent peroxidase activity.