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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Mol Microbiol. 2011 Jan 6;79(5):1194–1203. doi: 10.1111/j.1365-2958.2010.07500.x

Figure 4. Selenoprotein and non-selenoprotein Grxs reduce MsrAs with high substrate specificity.

Figure 4

(A) Reduction of selenoprotein Clostridium MsrA by Grxs. A. MsrA activity was measured according to concentrations of selenoprotein (cGrx1) and non-selenoprotein Grxs (cGrx2 and eGrx1) indicated. (B) Comparison of Grx-dependent selenoprotein MsrA activities with the corresponding DTT-dependent activity. The Grx-dependent activities were measured using 2.5 μg Grxs. (C) Reduction of the Cys mutant of selenoprotein Clostridium MsrA by Grxs. Activities of the Cys mutant of selenoprotein Clostridium MsrA, in which Sec is replaced with Cys, were analyzed in DTT- and Grx-dependent reactions. 2.5 μg Grxs were used in the Grx-dependent assays.