Table 2.

Mitochondrial inheritance and movement are impaired in arp2-1 and arc15-GFP mutants

Strain	Actin patch localization, % polarized	Mitochondrial morphology, % abnormal	Mitochondrial inheritance, % transfer	Mitochondrial movement
				Velocity (nm/sec)	% polarized
YPH499 (ARP2 wt)	53.1	22.9	ND	26.7  ± 6.5	51.4
YMW81 (arp2-1)	41.6	57.8	ND	7.2  ± 2.9	3.1
DNY226-4d (ARC15 wt)	89.2	22.8	89.2	17.1  ± 5.0	43.9
DNY226-4c (arc15-GFP)	89.6	75.2	57.4	9.6  ± 2.9	8.2

The conditions for temperature shift of the temperature-sensitive arp2-1 mutant and the corresponding wild-type cell strain were used as for Fig. 4. The actin cytoskeleton was scored as polarized if the cell showed no more than seven actin patches in the mother. Cells bearing long tubular mitochondria that aligned along the mother-bud axis were scored as normal for mitochondrial morphology. Cells bearing aggregated mitochondria were scored as abnormal. Inheritance represents the percentage of small buds that contain mitochondria. Long term shift of the arp2-1 strain produces severe actin disorganization and loss of cell viability. Therefore, inheritance was not determined (ND) for YPH499 and YMW81. Polarized movement refers to the percentage of motile mitochondria that showed a net displacement towards the bud. Velocity measurements were carried out as described in Supplementary Materials. The sample size for all measurements (n) was greater than 100.