Figure 1.

An hsp70-white transgene inserted within Dyrk3 displays variegating expression. (A) Schematic representation of Dyrk3 and a portion of Caps (modified from the RefSeq track of the UCSC Genome Browser). Bent arrows represent the transcription start sites (+1). Coding sequences are displayed as thick, blue boxes; transcribed, but not translated sequences are shown as thin, blue boxes and introns shown as thin, blue lines. The translation start sites (M) and the location of the P-element insertion (triangle) are indicated. (B) Eye phenotypes of strains containing the hsp70-white transgene. Females of the P-element insertion stock, 118E-15, were crossed to males containing mutations in known modifiers of PEV. Photographs of eyes from the parental stock and the resulting heterozygous progeny are shown. (C) Northern analysis of Dyrk3 and Caps. RNA was isolated from third instar larvae of y,w^67c23 (wild type) and Su(var)2-5, dSetdb1 or Su(var)3-9 mutants. Transcripts were detected by hybridization with an α-³²P labeled fragment for Dyrk3 or Caps. Detection of rp49 was used for loading control. Three arrowheads indicate alternatively spliced Dyrk3 transcripts. Results shown are representative of three experiments. (D) Northern analysis of Dyrk3 in a wild-type background and stock 118E-15. Arrow heads indicate wild-type transcripts, arrow indicates the truncated transcripts observed in the P-element-containing 118E-15 stock.