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. 2011 Apr 15;138(8):1501–1506. doi: 10.1242/dev.061309

Fig. 2.

Fig. 2.

Axin affects the dynamics of Notch endocytosis and traffic. (A-F′) Tracking of Notch in Drosophila wing discs with clones of Axin mutant cells (A-C) and overexpressing Axin, carrying a Myc tag, under the control of dpp-Gal4 (D-F) by pulsing with an antibody against the extracellular domain of Notch (red in merge) and chasing for 0 (A,D), 10 (B,E) and 30 (C,F) minutes. C′,F′ are higher magnifications of the boxed regions in C,F. The white lines indicate the localisation of Axin mutant clones (labelled by the absence of GFP, green channel in the lower panels) in A-F, and the domain of Axin overexpression (labelled by the anti-Myc staining, green channel in the lower panels) in D-F. In all cases, the maximum projection of an apical z-stack of confocal sections is shown. Proximal is to the left and posterior to the top. See Fig. S2 in the supplementary material for a quantification of the effect of the overexpression of Axin on Notch. Scale bars: 50 μm in F for A-F; 20 μm in C′ for C′,F′.