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. 2011 Apr 15;138(8):1521–1530. doi: 10.1242/dev.063537

Fig. 1.

Fig. 1.

Random distribution of S-phase cells in the VE at the time of AVE migration. (A) Stills taken from a time-lapse movie of a Hex-GFP mouse embryo showing how GFP-positive cells remain at the distal tip of the embryo (red arrow) when leading cells of the anterior visceral endoderm (AVE) domain are migrating. Time is shown in hours:minutes. (B) BrdU staining of a pre-distal Hex-GFP embryo. Nuc, TOPO-3 staining of DNA. (C) BrdU-incorporation index in pre-distal group embryos. Results are mean ± s.e.m.; n=5. (D) BrdU staining of a distal Hex-GFP embryo. Arrows indicate the embryonic/extra-embryonic boundary. (E) BrdU-incorporation index in distal group embryos for the distal visceral endoderm (DVE), embryonic VE (embVE) and extra-embryonic VE (exeVE) domains (see Fig. 2B). Results are mean ± s.e.m.; n=6. (F) BrdU staining of a tilted group Hex-GFP embryo. (G) BrdU-incorporation index of the anterior and posterior VE of tilted group embryos. Results are mean ± s.e.m.; n=11. (H) Relative fold difference in BrdU incorporation between the anterior and posterior VE regions of tilted group embryos. The red line indicates no relative difference between the two areas. The relative fold difference values 1-15 are 0.76, 1.24, 0.97, 0.57, 1.50, 1.29, 1.04, 0.89, 0.89, 0.98, 1.46, 2.60, 0.62, 1.08 and 1.18. (I) BrdU staining of a 6.25-dpc Hex-GFP embryo. (J) BrdU-incorporation index in the anterior and posterior VE of 6.25-dpc embryos. Results are mean ± s.e.m.; n=11. There were no significant differences between the anterior and posterior VE by Student's t-test for paired samples (P>0.05).