Table 2.
Examples of Polyelectrolyte Multilayers Used in Tissue Engineering Applications
| Research objective | Cationic/anionic PE | Cell type | Significant finding | References |
|---|---|---|---|---|
| Cell interaction | PLL/HA CHI/HA PLL/PGA |
Chondrocytes, chondrosarcoma | Crosslinking increased Young's modulus and cell adhesion, and spreading decreased enzymatic degradation | 149–151 |
| PEI/PAA SPS/PAH CHI/HA Amine Mod HA/HA |
Fibroblasts | Adhesion, proliferation, and cell morphology | 152,153 | |
| SPS/PAH CHI/HA PAA/PAH |
Endothelial cells | Adhesion, spreading, and viability | 154–157 | |
| Delivery of bioactive molecules | PLL/RNA PLL/PLGA CHI/HA PLL/HA |
Osteoblasts, COS-1; HeLa; HEK-293 | DNA or RNA incorporation to inhibit gene expression or as transfection vehicle | 158–161 |
| PLL/PGA PLL/HA PAH/PAA |
Cancer cell lines (CHO, melanoma, HT29); macrophages; fibroblasts | Embedded drugs, growth factors, or signaling molecules for cellular response modulation | 162–167 |
CHI, chitosan.