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Cell culture in single and multilayer (A) Schematic procedure of cell culture. (B) SEM morphology of single cells on single fibers in a single thin layer. (C) Fluorescent micrographs of cells on fibers in three layers of fibers after 7-day cell culture. The samples were stained with Alexa phalloidin for cytoskeletal actin (red) and counterstained with DAPI for nuclei (blue) of cells. Scale bar corresponds to 50 μm. Color images available online at www.liebertonline.com/teb.
