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. Author manuscript; available in PMC: 2011 Jul 7.
Published in final edited form as: Cell. 2011 Jan 7;144(1):79–91. doi: 10.1016/j.cell.2010.12.016

Figure 2.

Figure 2

Life-span analysis of tissue-specific complementation of rde-1 with cco-1 feeding RNAi. A. Tissues exposed to dsRNA from feeding RNAi initiate knockdown if rde-1 has been rescued in the corresponding tissue. Neighboring tissues are unable to initiate RNAi if rde-1 is absent. B. rde-1(ne219) mutants do not respond to cco-1 feeding RNAi. Animals fed bacteria harboring an empty vector (black line, mean 18.0 +/- 0.3 days), cco-1 RNAi (red line, mean 18.16 +/-0.4 days, p<0.4043). C. rde-1 rescued in the intestine (VP303) extends life span when fed cco-1 dsRNA producing bacteria. Animals fed vector only bacteria (black line, mean 14.7 +/- 0.6 days), cco-1 RNAi (green line, mean 22.0 +/- 0.2 days, p<.0001). D. rde-1 rescued in the body wall muscle (NR350.5) decreases life span when fed cco-1 dsRNA bacteria. Animals fed bacteria harboring empty vector (black line, mean 13.5 +/- 0.3 days), cco-1 RNAi (blue line, mean 11.8 +/- 0.3 days, p<0.0002. E. rde-1 rescued in the hypodermis (NR222) has no effect on life span when fed cco-1 dsRNA producing bacteria. Vector only (black line, mean 13.5+/- 0.3days), cco-1 RNAi (purple line, mean 14.3 +/- 0.4 days, p=0.148). F. Life-span extension by cco-1 feeding RNAi in the intestine of ges-1:;rde-1 rescued animals is independent of daf-16. Intestinal rde-1 rescued worms were fed empty vector (black line), mean 16.4+/- 0.6 days, daf-16 RNAi diluted 50% with empty vector (gold line, mean 16.3+/- 0.4days) or daf-16 diluted 50% with cco-1 RNAi (green19.9+/- 0.5 days, p<.0001). Please see Table S1 for all statistical analysis. G. Double transgenic animals carrying rab-3:;cco-1HP and ges-1:cco-1HP (blue line, mean life span 23.4 +/-0.6 days) did not live longer than either the rab-3∷cco-1HP (red line, mean life span 23.2 +/-0.6 days, p =.64) or the the ges1∷cco-1HP (green line, mean life span 22.9+/-0.6 days, p=.75) animals. sid-1 control (black line, mean life span 19.2+/-0.5 days).