Skip to main content
. 2011 Mar 22;6(3):e18186. doi: 10.1371/journal.pone.0018186

Figure 3. Analysis of the chemotactic and chemotaxis-antagonizing effects of pIP-10-AT.

Figure 3

(A) Chemotactic effect of pIP-10-AT in vitro. 293T cells were transfected with the indicated plasmids; 48 h later cell supernatant was collected and subjected to chemotaxis assays. (B) Competitive binding assay. CHO/mCXCR3 cells were incubated with FITC-IP-10 protein (20 µg/ml) and unlabeled competitor IP-10-AT protein (200-600 µg/ml). Unlabeled BSA was used as a negative control. Cells were washed extensively to remove unbound protein and analyzed by flow cytometry. Mean fluorescence intensities (MFI) are plotted as a function of increasing quantities of competitor proteins. Error bars represent three independent experiments. (C) Chemotactic and antagnizing effects of pIP-10-AT in vivo. Muscular sections at the injection sites were prepared 3 days after pIP-10 or pIP-10-AT intramuscular injection. (D) Recruited cell number in muscular tissues of mice receiving indicated plasmids. The results were presented as the mean ±SD of three separate experiments.