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Purification of A. pernix cytochrome c553.
| Steps | Total Protein (mg) |
c-type cytochrome | |
|---|---|---|---|
| Total (nmol) | Specific (nmol mg-1) | ||
| Membranes | 589 | 463 | 0.787 |
| DDM extract | 494 | 344 | 0.697 |
| DEAE-Toyopearl | 27.1 | 105 | 3.88 |
| Q-Sepharose | 21.1 | 30.8 | 1.46 |
| Hydroxyapatite | 2.00 | 15.1 | 7.55 |
