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. 2011 Mar 9;12:144. doi: 10.1186/1471-2164-12-144

Figure 5.

Figure 5

miRNA expression measurements by Fluidigm dynamic array and Affymetrix microarray. The raw intensity values of microarray data were transformed to log2 values for comparison to PCR Ct values generated by the qPCR platform. Gene expression differences between FF and FFPE were compared against 59 shared genes. Fold differences by the dynamic array (log2) were calculated by ΔΔCt method; ΔCt = (target miRNA log2 values-hsa-mir-16 log2 value), ΔΔCt = (fresh frozen ΔCt-FFPE ΔCt).