Table 1.
Comparison of gene expression measurements between Affymetrix microarray and Fluidigm 96.96 dynamic array.
| Platform | Sample† | Background | Signal to Noise | Positive Signal Intensity or Ct | Detection Rate (%) | Replicate Correlation | |||
|---|---|---|---|---|---|---|---|---|---|
| AVE | MAX | MIN | MAX | AVE | |||||
| Microarray (847 miRNA) |
FF#1a | 2.66 | 15.79 | 189 | 3.49 | 10530 | 503 | 33 | 0.997 |
| FF#1b | 2.66 | 11.68 | 185 | 1.73 | 10313 | 493 | 33 | ||
| FFPE#9a | 2.26 | 35.38 | 208 | 2.34 | 9331 | 470 | 35 | 0.996 | |
| FFPE#9b | 2.22 | 17.85 | 209 | 2.59 | 8724 | 464 | 35 | ||
| 96.96 dynamic array (94 miRNA) |
FF#1a | N/A | N/A | - | 7.9 | 22.4 | 16.7 | 91 | 0.954 |
| FF#1b | N/A | N/A | - | 5.0 | 22.7 | 16.2 | 91 | ||
| FFPE#9a | N/A | N/A | - | 5.9 | 35.9 | 19.5 | 85 | 0.956 | |
| FFPE#9b | N/A | N/A | - | 4.2 | 34.9 | 19.0 | 85 | ||
† Samples are as described in the method; N/A, not applicable.