Table 1.
Primers used in this study.
| Name | Sequence (5′ – 3′) | Description |
|---|---|---|
| PrecR-F | AACGCTTCATTAAGCGCTTT | To construct recR::cat mutant |
| PrecR-R | ACAAAGGCACTTCATGCAC | |
| recR-1 | GTTGAATCTCCTGGCACGAGTTTGATATTG | For recR complementation |
| recR-2 | ACGGCCACTATCGCATGGTTATAAGCATGC | |
| rdxA-L | CACAACCAAGTAATCGCATC | |
| rdxA-1 | CTCGTGCCAGGAGATTCAACCACAGCATGC | |
| rdxA-2 | AACCATGCGATAGTGGCCGTGTGGTAACAA | |
| rdxA-R | AGCGCCATTCTTGCAAGATG | |
| PrecO-F | CATGCGGAAATGGTAATGGC | To construct recO::aphA mutant |
| PrecO-R | GCTTGTTCTGTGTTTTCGCC | |
| PrecO-11 | CGAATCACCTAGTATCCGCTATGGCGTTTGCCATAG | |
| PrecO-22 | GATTCAGGAACTGATCGCATTAGAGCGTTCTGTCGC | |
| KanF-11 | CGGATACTAGGTGATTCGAGACGATAAATGCGTCGG | Forward primer for aphA cassette |
| KanR-22 | GCGATCAGTTCCTGAATCGACATCTAAATCTAGGTAC | Reverse primer for aphA cassette |
| D350P1 | TTATCGAGCTGTATGCGGAG | To construct IDS350 |
| D350P2 | TCCATACGATACCGGGTACCTGCCATCTTTCACAAAGATG | |
| D350P3 | CTCCGCATACAGCTCGATAA | |
| D350P4 | GGTACCCGGTATCGTATGGA | |
| Hp405-F | CTCCCTTACTCAACCCTAAC | To construct hp405::IDS100 and hp405::IDS350 |
| Hp405-R | CGTGAAGTGCAAGCTCACTC | |
| Hp405-11 | CGAATCACCTAGTATCCGCCATATCTTTCAAATACGGC | |
| Hp405-22 | GATTCAGGAACTGATCGCATACGCTAATCGCACACGGC | |
| RecR-F | CCTCATCTATAATGACCACA | To construct recR::IDS100 and recR::IDS350 |
| RecR-R | GAGATGAATTGAGTCGTCAG | |
| RecR-11 | CGAATCACCTAGTATCCGACCCAAATGATACGCCA | |
| RecR-22 | GATTCAGGAACTGATCGCGACTCAGTTTCGCTCTCA | |
| RecA-F | TTGCGCTCACATCATAAGTG | To construct recA::IDS100 and recA::IDS350 |
| RecA-R | CTTGATTGATCGCTTCAAGC | |
| RecA-11 | CGAATCACCTAGTATCCGGAGCCTGTAGAAATAGAGTC | |
| RecA-22 | GATTCAGGAACTGATCGCCTCTAATGAAGAGATCATGC |