Table 1.
Primers used for real time PCR and product size for myosin heavy-chains and calcium channels
| Target | Forward primer 5′ → 3′ | Reverse primer 5′ → 3′ | amplicon size (bp) |
|---|---|---|---|
| B-actin | TTCAACACCCCAGCCATGT | GTAGATGGGCACAGTGTGGGT | 120 |
| MHC-2x | GTACCACAGGGAAACTGGCTT | CTTGGTCATCAATGCTGGG | 222 |
| MHC-2a | GTCTGCCAACTTCCAGAAGC | CAGCTTGTTCAAATTCTCTCTGAA | 307 |
| MHC-2b | AAAGGTGGCCATTTACAAGCT | CAGCAGAGTTCAGACTTGTCAG | 146 |
| MHC-I | AAGTCCTCCCTCAAGCTCATGGC | ATTTTCCCGGTGGAGAGC | 135 |
| CaV1.1 | AGCTACCACCATGCTGATCC | CCCTCGCTTTCTGACTTTTG | 333 |
| RyR-1 | CAAGACCTGAGCTGAGACCC | CCCAATCTCAATTTCTCGGA | 242 |