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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Jan 20;1808(4):1140–1145. doi: 10.1016/j.bbamem.2011.01.008

Figure 3. Effects of additions of Cys66 and Cys298 to CL-PCFT, and substitutions of these residues with Ser in WT-PCFT, on protein expression and function.

Figure 3

Panel A: MTX influx was assessed in transfectants as described in Figure 2 and indicated as percentage of WT-PCFT. Data are the mean ± SEM from three independent experiments. Panel B: PCFT expression in the crude membrane fraction and biotinylated at the plasma membrane. Actin is the loading control for the crude membrane fraction. The Western blots are representative of two separate experiments.