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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Jan 20;1808(4):1140–1145. doi: 10.1016/j.bbamem.2011.01.008

Figure 4. Comparison of function and expression of PCFT mutants in which a variety, and variable number, of Cys residues have been re-introduced into the K422C-CL.

Figure 4

Panel A: MTX influx was assessed in transfectants as described in Figure 2 and is indicated as percentage of WT-PCFT. Data are the mean ± SEM from three independent experiments. The mutants are grouped according to the number of Cys residues and arranged from low to high activity within each group. All mutants contain the K422C mutation. “+C66” indicates a mutant in which native Cys66 was reintroduced into K422C-CL; +C151+C397” indicates a mutant with both native Cys 151 and 397 were simultaneously introduced into K422C-CL. Other mutants are designated in the same fashion. Panel B: PCFT in the crude membrane fraction and accessible at the plasma membrane. Actin is the loading control for the crude membrane fraction. The Western blot analysis is representative of two separate experiments.