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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: J Mol Biol. 2011 Feb 12;407(5):661–672. doi: 10.1016/j.jmb.2011.02.015

Fig. 3.

Fig. 3

Mismatch extension assays. End-labeled matched primers were annealed to the 38-mer RNA template (a) or DNA template (b) and extended by HIV-1 RT (H), MuLV RT (M) and R2 RT (R) at 37 °C for 15 min with 250 μM dNTPs. The primers corresponded to a matched 17-mer [left panels in (a) and (b)], a 16-mer with a terminal G/T mismatched annealed to an RNA template [right panel in (a)], and a 19-mer with a terminal C/A mismatched annealed to a DNA template [right panel in (b)]. Reaction products were analyzed on 14% polyacrylamide-urea denaturing gels. Again the terminal transferase activity of R2 RT generated products longer than full length.