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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: J Mol Biol. 2011 Feb 12;407(5):661–672. doi: 10.1016/j.jmb.2011.02.015

Fig. 4.

Fig. 4

Mismatch extension assays with one, two or three consecutive terminal mismatches. Protein activities were equilibrated with a 17-mer match primer on an RNA template as in Figure 3 (left panel). The extension reactions were repeated with a 16-mer containing one terminal mismatched nucleotide (G/T), a 17-mer with two terminal mismatches (GA/TC), or an 18-mer with three terminal mismatches (GAU/TCG). The mismatches between the primer and template are shown above the gel photographs and indicated with dots. The reactions were analyzed on 14% polyacrylamide-urea denaturing gels.