Fig. 5.

Mutations generated by R2 RT in the lacZ gap-filling reactions. The wild type sequence of the M13 viral (+) template strand is shown and corresponds to the strand used as template in the RT gap-filling reactions. Position +1 is the first transcribed base while the ATG initiation codon for the lacZ open reading frame is underlined. Triangles represent one base deletions. Substitutions and deletions shown above the sequence were obtained from two independent filling and transformation experiments using R2 RT. Reaction 1 generated two T to A transversions at position 87, two G to A transitions at position 145 and a one base deletion in the run of Cs at position 106–108. All other mutations were generated by reaction 2. Substitutions and deletions shown below the primary sequence are mutations generated by HIV-1 RT. Those without circles were obtained in this report, while those that are circled are HIV-1 mutation hot spots identified in previous studies.¹⁰,³⁶–³⁸