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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: J Mol Biol. 2011 Feb 12;407(5):661–672. doi: 10.1016/j.jmb.2011.02.015

Table 1.

Error rate of R2 RT and HIV-1 RT in lacZ gap-filling reactions.

Total number of plaques Mutant plaques Mutant frequency
HIV-1 RT Exp. 1 4499 102 2.26 × 10−2
Exp. 2 4316 93 2.15 × 10−2
R2RT Exp. 1 4062 70 1.72 × 10−2
Exp. 2 4601 88 1.91 × 10−2

Experiments 1 and 2 correspond to independent gap-filling reactions. In each experiment total plaques and mutant plaques were combined from two separate transformation reactions. Background mutation frequency (absence of gap-filling) was <5 × 10−5.