(A) PCR assay of representative strains from Kolkata using primer set PAI-1S and PAI-1AS amplifying part of HP0519, HP0520, and HP0521. Lane 1 represents 26695 while lanes 2–12 represent different clinical isolates from Kolkata.6
(B) PCR assay of the same set of strains as in (3A) using primer set PAI-3S-PAI-3AS amplifying part HP0522 and HP0523.
(C) PCR assay of representative strains using primers PAI-1S and PAI-6AS designed from HP0519 and HP0524 gene of 26695, respectively. Lane 1 represents 26695, lanes 2–10 represent the strains that were negative for HP0522-523 by PCR, lane 11 is a 1-kb marker, and lane 12 indicates a cag PAI-negative strain as a negative control.