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. 2001 Mar 13;98(6):3180–3184. doi: 10.1073/pnas.051003498

Figure 2.

Figure 2

Distribution of ubiquitinated proteins and Lyn upon activation with multivalent antigen. (A) RBL cells were transfected with a HA-tagged ubiquitin chimera. Sensitized RBL cells were activated (+Ag) or not (−Ag) with DNP-BSA for less than 5 min at room temperature and then fixed and processed for immunolabeling with FITC-conjugated anti-DNP and polyclonal anti-HA antibodies plus tetramethyl isothiocyanate (TRITC)-conjugated anti-rabbit antibodies. Two magnifications are presented for activated cells. The red channel (Left), the green channel (Center), and the merged channels (Right) are shown. (B) RBL cells were activated, fixed, and immunolabeled with FITC-conjugated anti-DNP and polyclonal anti-Lyn antibodies plus TRITC-conjugated anti-rabbit antibodies (with or without MeCD treatment) or with polyclonal anti-HA antibodies plus FITC-conjugated anti-rabbit antibodies and monoclonal anti-Lyn antibodies plus TRITC-conjugated anti-rat antibodies. Only merged pictures are shown. (Bars = 10 μm.)