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Distribution of ubiquitinated proteins and Cbl upon activation with multivalent antigen with or without MeCD treatment. Activated RBL cells were immunolabeled with polyclonal anti-Cbl antibodies plus TRITC-conjugated anti-rabbit antibodies. Costaining was performed by using either FITC-conjugated anti-DNP antibodies or monoclonal anti-HA primary plus FITC-conjugated secondary antibodies. Merged pictures are shown. (Bars = 10 μm.)
