Skip to main content
. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Exp Hematol. 2011 Feb 1;39(4):473–485.e4. doi: 10.1016/j.exphem.2011.01.009

Figure 3.

Figure 3

The Flt3-ITD increases p53 induction. A) Alkaline comet assays were performed after cells were treated with the indicated chemotherapy for four hours. Ten to twelve comets were quantitated per condition and the experiment was carried out 3 times. A representative result is shown. B) Western blot for murine p53 and p21. Cells were exposed to the indicated amounts of doxorubicin for 4 hours, harvested and lysates analyzed for p53 and p21. D) Q-PCR results for p53 transcriptional targets p21 and MDM2. Cells were exposed to the indicated chemotherapies for 4 hours and total RNA was isolated. QPCR was performed and relative transcript amounts as compared to an actin control were plotted.