Figure 5. Neuroprotective effect of nanozyme-loaded BMM in an MPTP model of PD.
MPTP-intoxicated C57Bl/6 mice (18 mg/kg) were intravenously injected with saline (second bar), nanozyme alone (third bar), macrophages loaded with nanozyme (5×106 cells/mouse/100μl) (fourth bar), or empty macrophages (fifth bar). Healthy non-intoxicated animals were used as control group (first bar). Seven days after treatment the animals were sacrificed; brain slices were stained for tyrosine hydroxylase-positive nigral dopaminergic neurons (A). Results from N = 5 animals per group demonstrate a significant loss of nigrostratial neurons in MPTP treated mice, which is prevented by adoptive transfer of macrophages loaded with nanozyme (B). No significant neuroprotective effect was detected after treatment with nanozyme alone, or empty macrophages. Values are means ± SEM, and P < 0.05 compared with asaline; bMPTP; cMPTP+macrophage/nanozyme. Used with permission 71.