Table 1.
Sample and laboratory diagnosis of fungal keratitis
| Patient | Sample | Date | Stain | Culture | Identification culture | Fungal PCR | Molecular identification | Topical/oral treatment |
|---|---|---|---|---|---|---|---|---|
| 1 | Corneal scrape | 15/01/2000 | Septate hyphae | Positive (2 days) | Aspergillus fumigatus | Positive | Aspergillus fumigatus | Amphotericin B/fluconazole |
| 2 | Corneal scrape | 06/10/2000 | Negative | Negative | Positive | Aspergillus niger | Amphotericin B | |
| 3 | Corneal scrape | 12/02/2001 | Negative | Positive (7 days) | Alternaria alternata | Positive | Alternaria alternata | Amphotericin B/fluconazole |
| 4 | Corneal scrape | 15/06/2001 | Hyphae | Positive (6 days) | Scedosporium apiospermum | Positive | Scedosporium apiospermum | Natamicin/voriconazole |
| 5 | Corneal scrape | 19/03/2002 | Yeast | Positive (1 day) | Candida albicans | Positive | Candida albicans | Amphotericin B/fluconazole |
| 6 | Corneal scrape | 02/08/2002 | Hyphae | Positive (7 days) | Alternaria sp. | Positive | Alternaria infectoria | Amphotericin B/fluconazole |
| 7 | Corneal scrape | 18/11/2002 | Broken hyphae | Negative | Positive | Aspergillus oryzae | ||
| 8 | Corneal scrape | 18/02/2003 | Septate hyphae and conidia | Positive (1 day) | Fusarium sp. | Positive | Fusarium oxysporum | Amphotericin B/econazole |
| 9 | Corneal biopsy | 03/08/2003 | Negative | Negative | Positive | Candida parapsilosis | ||
| Corneal biopsy | 10/08/2003 | Yeast | Positive (2 days) | Candida parapsilosis | Positive | Candida parapsilosis | Amphotericin B/voriconazole | |
| 10 | Corneal scrape | 07/07/2004 | Septate hyphae | Positive (1 day) | Aspergillus niger | Positive | Aspergillus niger | Amphotericin B |
| 11 | Corneal scrape | 20/05/2005 | Negative | Negative | Positive | Candida famata | Amphotericin B/itraconazole | |
| 12 | Corneal scrape | 03/08/2005 | Yeast | Negative | Positive | Candida parapsilosis | Amphotericin B/voriconazole | |
| 13 | Corneal scrape | 13/10/2005 | Negative | Negative | Negative | |||
| Corneal scrape | 20/10/2005 | Yeast and hyphae | Negative | Positive | Candida sake | Natamicin/itraconzaole | ||
| 14 | Corneal scrape | 02/02/2007 | Negative | Negative | Negative | |||
| Corneal scrape | 02/02/2007 | Negative | Negative | Positive | Fusarium solani | |||
| Corneal biopsy | 07/02/2007 | Hyphae | Positive (7 days) | Fusarium solani | Positive | Fusarium solani | Voriconazol/voriconazole | |
| 15 | Corneal scrape | 05/03/2007 | Septate hyphae | Negative | Positive | Pyrenochaeta keratinophila | ||
| Corneal biopsy | 13/03/2007 | Septate and branched hyphae | Positive (6 days) | Unidentified fungi | Positive | Pyrenochaeta keratinophila | Natamicin/itraconazole | |
| 16 | Corneal scrape | 07/04/2008 | Septate hyphae | Positive (2 days) | Sterile mycelium | Positive | Paecilomyces sp. | |
| Corneal scrape | 03/06/2008 | Septate hyphae | Negative | Positive | Paecilomyces sp. | |||
| Cornea | 06/06/2008 | Septate and branched hyphae | Positive (35 days) | Sterile mycelium | Positive | Paecilomyces sp. | Natamicin/itraconazole | |
| 17 | Corneal scrape | 19/08/2008 | Negative | Positive (6 days) | Unidentified fungi | Positive | Scedosporium apiospermum | Voriconazole/voriconazole |
| 18 | Corneal scrape | 04/02/2009 | Yeast | Positive (1 day) | Candida sp. | Positive | Candida dublinensis | Fluconazole/fluconazole |
| 19 | Corneal biopsy | 05/06/2009 | Septate and branched hyphae | Positive (2 days) | Fusarium solani | Positive | Fusarium solani | Natamicin/voriconazole |
| 20 | Corneal scrape | 23/06/2009 | Negative | Positive (2 days) | Candida sp. | Positive | Candida parapsilosis | Fluconazole/fluconazole |