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. 2011 Mar 15;435(Pt 1):113–125. doi: 10.1042/BJ20101734

Figure 1. Characterization of a K562 cell-line derivative that expresses BASP1.

Figure 1

(A) V-K562 and B-K562 cells were used to prepare nuclear (N) and cytoplasmic (C) extracts and immunoblotting was performed with the antibodies indicated. (B) Anti-FLAG antibodies were used in immunoprecipitation (IP) with nuclear extracts prepared from V-K562 and B-K562 cells. The samples were then immunoblotted with anti-WT1 or anti-BASP1 antibodies. (C) An equal number of V-K562 and B-K562 cells were grown over a 4 day period and each day the cells were counted. The y-axis of the graph shows the fold increase in cell number during the period. Immunoblots of WT1, BASP1 and β-tubulin are shown in the right-hand panels. Values are means±S.D. for six independent experiments. P<0.01 by Student's t test.