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. 2011 Mar 15;435(Pt 1):113–125. doi: 10.1042/BJ20101734

Figure 5. WT1 is required for the altered PMA-dependent differentiation programme of B-K562 cells.

Figure 5

(A) V-K562 or B-K562 cells were treated with 100 nM PMA for the times indicated and then whole-cell extracts were prepared and subjected to immunoblotting with anti-WT1 antibodies. (B) V-K562 and B-K562 cells were transfected with either control or WT1 siRNA. At 24 h later, whole-cell extracts were prepared and immunoblotted with the antibodies indicated. (C) V-K562 or B-K562 cells were transfected as described in (B) along with a vector driving expression of GFP, and 24 h later were treated with 100 nM PMA or vehicle control. At 48 h later, the cells were imaged. Quantification of the arborized cells is shown in the lower panel. Values are means±S.D. for six experiments (P<0.05 by Student's t test).