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Pattern of adherence to HEp-2 (A and B) and IPEC-J2 (C and D) cells of EHEC O157:H7 strain 86-24 grown in LB (A and C) and BHIN (B and D). The cells were stained with Giemsa and viewed by light microscopy (×400) after the 6-h adherence assay. Bacteria adhered to IPEC-J2 cells in clusters that appeared localized but not in the classic pattern (arrows). Adherence was more extensive for bacteria cultured in BHIN than for those cultured in LB for both HEp-2 and IPEC-J2 cells.
