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Recovery of IL-6 synthesis by delaying the time between LPS challenge and HNE treatment. Western blot for IL-6 from THP-1 macrophage cell lysate. Cells were pretreated with 10 μM HNE or the vehicle control for 30 min followed by washing with PBS. Following washout of the electrophile, cells were challenged with LPS/IFN-γ after an incubation time of 0, 0.5, or 3 h. The cell lysate was probed with either anti-IL-6 or anti-actin antibodies.
