Table 2. Half-Lives and Second Order Rate Constants for the Reaction of HNE, ONE, and their Analogues with N-Acetyl Cysteine.
| electrophile | half-life (s)a | k (M−1 s−1)b |
|---|---|---|
| HNE | 732 (±44) | 1.22 (±0.08) |
| HNEA | NR | NR |
| COOH-HNE | 1213 (±116) | 0.79 (±0.11) |
| COOMe-HNE | 794 (±48) | 1.18 (±0.08) |
| ONE | 7.1 (±0.6) | 238 (±13) |
| ONEA | 580 (±40) | 1.72 (±0.28) |
| COOH-ONE | 13.9 (±0.7) | 169 (±7) |
| COOMe-ONE | 7.1 (±0.5) | 301 (±9) |
a
Half-life determined in the presence of 1 mM NAC in phosphate buffer (50 mM, pH 7.4) at 37 °C.
b
Rate constant determined in the presence of varying concentrations of NAC in phosphate buffer (100 mM, pH 7.4) at 37 °C.