Table 2.
Sensitivity and calibration ranges in different matrices
| ARG | 15N4-ARG | CIT | ADMA | SDMA | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Matrix | LLOQ (μM) | Calibration range (μM) | LLOQ (μM) | Calibration range (μM) | LLOQ (μM) | Calibration range (μM) | LLOQ (μM) | Calibration range (μM) | LLOQ (μM) | Calibration range (μM) |
| Mobile Phase B |
0.1 | 0.1 – 20 | 0.1 | 0.1 – 20 | 0.02 | 0.02 – 1 | 0.010 | 0.010 – 0.500 | 0.010 | 0.010 – 0.500 |
| Cell incubation medium |
0.1 | 0.1 – 20 | 0.1 | 0.1 – 20 | 0.02 | 0.02 – 1 | 0.010 | 0.010 – 0.500 | 0.010 | 0.010 – 0.500 |
| Cell lysate, LGLAa |
5 | 5 – 200 | 0.1 | 0.1 – 20 | 0.1 | 0.1 – 5 | 0.025 | 0.025 – 1.000 | 0.010 | 0.010 – 0.500 |
| Cell lysate, HGHAb |
5 | 5 – 200 | 0.1 | 0.1 – 20 | 0.1 | 0.1 – 5 | 0.025 | 0.025 – 1.000 | 0.010 | 0.010 – 0.500 |
| Plasma | 5 | 5 – 200 | 0.1 | 0.1 – 20 | 2 | 2 – 100 | 0.025 | 0.025 – 1.000 | 0.010 | 0.010 – 0.500 |
| Urine | 5 | 5 – 200 | 0.1 | 0.1 – 20 | 0.2 | 0.2 – 10 | 0.025 | 0.025 – 1.000 | 0.100 | 0.100 – 5.00 |
a
Cell lysate obtained from EA.hy926 cells after grown in low glucose and low arginine condition
b
Cell lysate obtained from EA.hy926 cells after grown in high glucose and high arginine condition.