. Author manuscript; available in PMC: 2012 Mar 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Jan 14;879(7-8):467–474. doi: 10.1016/j.jchromb.2011.01.006

Table 3.

Intra- and inter- day accuracy and precision of QC samples at the lowest concentration for 5 analytes in 6 media (n=5 each). Accuracy is determined as the percentage deviation of calculated concentrations from the nominal concentrations; Precision is determined as the coefficient of variation (CV) of the 5 measurements. Additional data using higher concentrations can be seen in Supplementary Table 3.

A. Intraday variability
	Matrix	Mobile phase B	Cell incubation medium	Cell lysate, LGLA^a	Cell lysate, HGHA^b	Rat plasma	Rat urine
ARG	Concentration (μM)	0.2	0.2	8	10	8	8
	Accuracy (%)	−8.30	−1.80	8.80	6.20	−0.33	12.4
	Precision (%)	3.42	11.1	3.62	4.18	2.59	1.77

¹⁵N₄-ARG	Concentration (μM)	0.2	0.2	0.2	0.2	0.2	0.2
	Accuracy (%)	−1.90	−8.80	2.20	−3.60	−9.00	10.9
	Precision (%)	2.68	4.11	2.60	6.16	2.46	5.73

CIT	Concentration (μM)	0.04	0.04	0.15	0.2	4	0.4
	Accuracy (%)	−2.5	3.15	−5.20	7.40	9.70	10.8
	Precision (%)	5.46	6.44	8.66	2.19	2.38	4.98

ADMA	Concentration (μM)	0.02	0.02	0.04	0.05	0.04	0.04
	Accuracy (%)	−7.50	4.30	10.10	−6.40	2.05	−12.8
	Precision (%)	4.67	5.72	4.70	10.4	8.50	9.57

SDMA	Concentration (μM)	0.02	0.02	0.02	0.025	0.02	0.2
	Accuracy (%)	−3.4	−1.30	8.70	4.96	0.50	7.30
	Precision (%)	5.4	6.33	2.55	10.8	8.56	11.2

B. Interday variability
	Matrix	Mobile phase B	Cell incubation medium	Cell lysate, LGLA^a	Cell lysate, HGHA^b	Rat plasma	Rat urine
ARG	Concentration (μM)	0.2	0.2	8	10	8	8
	Accuracy (%)	4.60	3.50	−1.78	−4.88	6.90	7.90
	Precision (%)	13.6	11.7	8.35	8.50	9.27	5.09

¹⁵N₄-ARG	Concentration (μM)	0.2	0.2	0.2	0.2	0.2	0.2
	Accuracy (%)	4.30	−5.50	0.70	3.60	−1.50	9.70
	Precision (%)	7.97	6.89	3.59	5.60	7.11	4.52

CIT	Concentration (μM)	0.04	0.04	0.15	0.2	4	0.4
	Accuracy (%)	0.85	7.30	−1.73	7.10	8.30	4.10
	Precision (%)	6.34	5.51	9.26	2.32	3.81	9.60

ADMA	Concentration (μM)	0.02	0.02	0.04	0.05	0.04	0.04
	Accuracy (%)	−3.30	−7.00	1.20	−1.12	3.25	−12.1
	Precision (%)	2.09	4.11	11.3	14.4	8.90	4.59

SDMA	Concentration (μM)	0.02	0.02	0.02	0.025	0.02	0.2
	Accuracy (%)	−2.60	−7.00	−0.20	5.52	1.30	10.3
	Precision (%)	9.74	4.81	11.1	9.24	11.4	10.5

Cell lysate obtained from EA.hy926 cells after grown in low glucose and low arginine condition

Cell lysate obtained from EA.hy926 cells after grown in high glucose and high arginine condition.