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. Author manuscript; available in PMC: 2011 Mar 23.
Published in final edited form as: Mol Microbiol. 2009 Nov 2;74(5):1198–1210. doi: 10.1111/j.1365-2958.2009.06928.x

Figure 3. Purified MBP-CbiZ cleaves Cbi and pseudoCbl, but not Cbl.

Figure 3

Amidohydrolase activity of MBP-CbiZ (expressed as nanomoles of Cby min-1 mg-1 protein) is shown. Error bars of one standard deviation are indicated. Unless indicated, reactions contained CHES buffer (50 mM, pH 10), DTT (1 mM), MBP-CbiZ (2 μg), and AdoCbi (30 μM) and were incubated for 15 min at 50°C.

A. Activity of MBP-CbiZ as a function of pH at 30°C. B. Activity of MBP-CbiZ as a function of temperature at pH 10. C. Activity of MBP-CbiZ as a function of time and enzyme concentration. D. Activity of MBP-CbiZ with different corrinoid substrates (30 μM). Reactions were incubated for 10 min.