Table 2.

Strains and plasmids used in this study.^a

Strain or plasmid	Marker(s)^b	Relevant genotype	Reference or source
R. sphaeroides strains:
JE8777		2.4.1 wild-type	Lab collection
JE10754		ΔcobB	(Gray & Escalante-Semerena, 2009)
JE10755		ΔcobB ΔcbiZ	(Gray & Escalante-Semerena, 2009)
JE10778	Tc^R	ΔcobB / pRK404
JE10779	Tc^R	ΔcobB ΔcbiZ / pRK404
JE10781	Tc^R	ΔcobB ΔcbiZ / pRsCBIZ6
JE11661	Sp^R Sm^R	ΔcobB bluB∷aadA
JE11662	Sp^R Sm^R	ΔcobB ΔcbiZ bluB∷aadA
JE11805	Sp^R Sm^R Tc^R	ΔcobB bluB∷aadA / pRK404
JE11806	Sp^R Sm^R Tc^R	ΔcobB bluB∷aadA / pBLUB19
JE11807	Sp^R Sm^R Tc^R	ΔcobB ΔcbiZ bluB∷aadA / pRK404
JE11808	Sp^R Sm^R Tc^R	ΔcobB ΔcbiZ bluB∷aadA / pBLUB19
JE11821	Sp^R Sm^R Tc^R	ΔcobB ΔcbiZ bluB∷aadA / pRsCBIZ6
S. enterica strains:
TR6583		metE205 ara-9 derivative of strain LT2	K. Sanderson via J.R. Roth
JE8248		metE205 ara-9 cobS1313	lab collection
E. coli strains:
DH5α/F’		F’ / endA1 hsdR17(r_k^-m_k⁺) glnV44 thi-1 recA1 gyrA(Nal^R) relA1 Δ(lacIZYA-argF)U169 deoR (ϕ80dlacΔ(lacZ)M15)	(Woodcock et al., 1989, Raleigh et al., 1989)
S17-1		recA pro hsdR RP4-2-Tc∷Mu-Km∷Tn7	(Simonet al., 1983)
BL21(DE3)		F^-ompT gal dcm lon hsdSB(r_B^-m_B^-) λ(DE3 [lacl lacUV5-T7 gene 1 ind1 sam7 nin5])	Novagen
Plasmids:
pGEM^®-T Easy	Ap^R	TA cloning vector	Promega
pK18mobsacB	Km^R Suc^S	suicide vector for allelic exchange	(Schafer et al., 1994)
pRK404	Tc^R	shuttle vector with lac promoter	(Ditta et al., 1985)
pSRA2	Ap^R Sp^R Sm^R	aadA⁺	(Frigaard et al., 2004)
pKLD66	Ap^R	TEV protease-cleavable maltose-binding protein / His₆ tag overexpression vector	(Rocco et al., 2008)
pRsCBIZ3	Ap^R	R. sphaeroides cbiZ⁺ translational fusion to maltose-binding protein / His₆ tag for protein purification
pRsCBIZ6	Tc^R	R. sphaeroides cbiZ⁺
pBLUB19	Tc^R	R. sphaeroides bluB⁺
pBLUB20	Ap^R	R. sphaeroides bluB⁺ region
pBLUB21	Ap^R	ΔbluB for R. sphaeroides
pBLUB23	Ap^R Sp^R Sm^R	bluB∷aadA for R. sphaeroides
pBLUB24	Km^R Suc^S Sp^R Sm^R	bluB∷aadA for R. sphaeroides

Unless otherwise indicated, all strains and plasmids were constructed during the course of these studies.

Tc^R, tetracycline resistance; Sp^R, spectinomycin resistance; Sm^R, streptomycin resistance; Ap^R, ampicillin resistance; Kn^R, kanamycin resistance; Suc^S, sucrose sensitivity.