Table 2.
ATGL and CGI-58 expression in human adipose tissue and culture
| ATGL* | CGI-58* | LPL* | Leptin* | |
|---|---|---|---|---|
| Surgical adipose tissue depots (n=14) | ||||
| SAT | 2.45 ± 0.41 | 2.68±0.43 | 1.35±0.21 | 1.74±0.21 |
| VAT | 1.36 ± 0.28 a | 3.12±0.32 | 0.75±0.21a | 0.68±0.12a |
|
| ||||
| Cell (n=2)/tissue (n=3) | ||||
| Whole adipose tissue | 2.24±0.79 | 1.40±0.22 | 5.09±1.84 | 3.65±1.11 |
| Adipocytes from adipose tissue | 4.14±0.52 | 1.33±0.12 | 5.00±0.65 | 3.44±0.58 |
| Stromal fraction from adipose tissue | 0.18±0.04 | 1.12±0.17 | 1.01±0.27 | 0.20±0.05 |
| Adipocytes from SGBS preadipocytes | 4.53±0.32 | 2.22±0.14 | 7.44±0.10 | 0.16±0.01 |
| Cultured SGBS preadipocytes | 0.13±0.03 | 0.35±0.07 | NE | NE |
| Skeletal muscle | 0.33±0.03 | 0.46±0.09 | 0.22±0.08 | NE |
Data are mean ± SE.
*
The RNA was pooled from all the cDNA samples to generate a standard curve. Therefore, the data are expressed relative to each other.
a
p<0.02 vs. SAT. NE: no expression.