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. Author manuscript; available in PMC: 2011 Mar 23.
Published in final edited form as: Apoptosis. 2010 Dec;15(12):1444–1452. doi: 10.1007/s10495-010-0544-2

Figure 1. RAIDD CARD and RAIDD DD interact with caspase-2 CARD and PIDD DD respectively.

Figure 1

A. Schematic model of PIDDosome assembly. RAIDD that contains both CARD at N-terminus and DD at C-terminus works as adaptor between Caspase-2 and PIDD. RAIDD interacts to Caspase-2 via CARD-CARD interaction and PIDD via DD-DD interaction. B. Co-expression and His-tag pull-down of GST-caspase-2 CARD by His-tagged RAIDD CARD. Lane1: Marker; Lane2: Supernatant of the cell lysate; Lane3: Pellets of the cell lysate; Lane4: Flow through after incubation with Ni-NTA beads; Lane5: Imidazole eluted fraction. C. Native-PAGE for RAIDD DD (Lane 1), PIDD DD (Lane 2), and the mixed sample (Lane 3). Complex band and excess PIDD DD are appeared.