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. Author manuscript; available in PMC: 2011 Mar 23.
Published in final edited form as: Apoptosis. 2010 Dec;15(12):1444–1452. doi: 10.1007/s10495-010-0544-2

Figure 2. Full-length RAIDD failed to interact with neither Caspase-2 CARD nor PIDD DD.

Figure 2

A. Gel filtration chromatogram and fractions of full-length RAIDD. B. Co-expression and His-tag pull-down of GST-caspase-2 CARD by His-tagged full-length RAIDD. Lane1: Marker; Lane2: Supernatant of the cell lysate; Lane3: Pellet of the cell lysate; Lane4: Flow through after incubation with Ni-NTA beads; Lane5: Imidazole eluted fraction. Flow-through contains unbounded GST-caspase-2 CARD. Only RAIDD is present at the eluted fraction. C. Gel-filtration profile of the mixture of full-length RAIDD and PIDD DD. SDS-PAGE of the peak fraction is shown.